MersV1, Main, Exploration, bibRecord, 002B62

Evaluating the quality of data from DNA microarray measurements.

Identifieur interne : 002B62 ( Main/Exploration ); précédent : 002B61; suivant : 002B63

Evaluating the quality of data from DNA microarray measurements.

Auteurs : Lili Wang [États-Unis] ; A K Gaigalas ; M B Satterfield ; M. Salit ; Y. Zong ; J. Noble

Source :

Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2007.

RBID : pubmed:17984517

Descripteurs français

KwdFr :
- Calibrage, Carbocyanines (métabolisme), Humains, Oligonucléotides, Spectrométrie de fluorescence, Séquençage par oligonucléotides en batterie ().
MESH :
- métabolisme : Carbocyanines.
- Calibrage, Humains, Oligonucléotides, Spectrométrie de fluorescence, Séquençage par oligonucléotides en batterie.

English descriptors

KwdEn :
- Calibration, Carbocyanines (metabolism), Humans, Oligonucleotide Array Sequence Analysis (methods), Oligonucleotides, Spectrometry, Fluorescence.
MESH :
- chemical , metabolism : Carbocyanines.
- methods : Oligonucleotide Array Sequence Analysis.
- Calibration, Humans, Oligonucleotides, Spectrometry, Fluorescence.

Abstract

Gene expression technology offers great potentials to generate new insights into human disease pathogenesis; however, the data quality remains a major obstacle for realizing its potentials. In the present study 60-mers oligonucleotide target immobilized on coated glass slides were utilized as a model system to investigate parameters, such as target concentration, retention, signal linearity, and fluorescence properties of fluorophores, which likely affect the quality of microarray results. An array calibration slide was used to calibrate an Axon GenePix 4000A scanner and ensure the dynamic range of the instrument. The work is a first step toward our goal of quantitative gene expression measurements.

DOI: 10.1007/978-1-59745-303-5_6
PubMed: 17984517

Affiliations:

Le document en format XML

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<author><name sortKey="Zong, Y" sort="Zong, Y" uniqKey="Zong Y" first="Y" last="Zong">Y. Zong</name>
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<front><div type="abstract" xml:lang="en">Gene expression technology offers great potentials to generate new insights into human disease pathogenesis; however, the data quality remains a major obstacle for realizing its potentials. In the present study 60-mers oligonucleotide target immobilized on coated glass slides were utilized as a model system to investigate parameters, such as target concentration, retention, signal linearity, and fluorescence properties of fluorophores, which likely affect the quality of microarray results. An array calibration slide was used to calibrate an Axon GenePix 4000A scanner and ensure the dynamic range of the instrument. The work is a first step toward our goal of quantitative gene expression measurements.</div>
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Serveur d'exploration MERS

Evaluating the quality of data from DNA microarray measurements.

Evaluating the quality of data from DNA microarray measurements.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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